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| Registros recuperados: 62 | |
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| Masson, Daniel; Ratiskol, Gilles; Burgeot, Thierry; Margat, Sylvie. |
| Les secteurs conchylicoles atteints par les efflorescences phytoplanctoniques toxiques sont fermés par l'Administration dès que le taux de contamination devient dangereux pour la consommation humaine. Cette fermeture peut durer plusieurs mois empêchant les conchyliculteurs de commercialiser leurs produits, ce qui a des conséquences économiques parfois graves. Il n'existe jusqu'à présent aucune possibilité technique de décontaminer ces coquillages en grande quantité et dans des délais économiquement viables. L'étude entreprise visait à mettre au point une technique permettant aux professionnels de décontaminer des coquillages rapidement (quinze jours au moins) en milieu contrôlé (bassins). L'hypothèse testée était que des coquillages placés dans un milieu... |
| Tipo: Text |
Palavras-chave: Coquillages; Décontamination; Toxicité; Phytoplancton. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00134/24499/22527.pdf |
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| Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean. |
| Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All... |
| Tipo: Text |
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| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf |
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| Burgeot, Thierry; Fillon, Alain; Ratiskol, Gilles; Vayne, Jean-jacques. |
| La synthèse des informations collectées en 1988 en Charente Maritime, en matière de développement microalgaux a pour but de mettre en évidence, à partir des données recueillies au cours d'une année, des tendances et niveaux de contaminations phytoplanctoniques, ainsi qu'une certaine identité du secteur surveillé. L'année 1988 est marquée par une efflorescence monospécifique de Gymnodinium sp. en période estivale et par ['apparition du Dinophysis à des concentrations supérieures au seuil toxique sans qu'aucune toxicité ne soit décelée. Le réseau de suivi confirme, à travers la bonne représentativité des stations de prélèvement, la spécificité des pertuis charentais. |
| Tipo: Text |
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| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00119/23025/20856.pdf |
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| Burgeot, Thierry; Fillon, Alain; Ratiskol, Gilles; Vayne, Jean-jacques. |
| La synthèse des informations collectées en 1988 en Charente Maritime, en matière de développement microalgaux a pour but de mettre en évidence, à partir des données recueillies au cours d'une année, des tendances et niveaux de contaminations phytoplanctoniques, ainsi qu'une certaine identité du secteur surveillé. L'année 1988 est marquée par une efflorescence monospécifique de Gymnodinium sp. en période estivale et par l'apparition du Dinophysis à des concentrations supérieures au seuil toxique sans qu'aucne toxicité ne soit décelée. Le réseau de suivi confirme, à travers la bonne représentativité des stations de prélèvement, la spécificité des pertuis charentais |
| Tipo: Text |
Palavras-chave: Microalgues; Pertuis charentais; Efflorescences monospécifiques. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00443/55496/57039.pdf |
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| Galgani, Francois; Bocquene, Gilles; Truquet, Philippe; Burgeot, Thierry; Chiffoleau, Jean-francois; Claisse, Didier. |
| The establishment of a network to assess pollutant effects is a current concern of international bodies such as the North Sea Task Force, the International Council for the Exploration of the Sea and the Intergovernmental Oceanographic Commission. With respect to the French coasts, three major considerations have been defined : the choice of monitoring areas ; the determination of a target species according to different criteria such as wide distribution, limited migration and contact with pollutant-rich sediments; and the selection of suitable parameters. This paper concerns the strategy adopted for the French coast. Ethoxyresorufin-O-deethylase, a specific cytochrome P450-dependent monooxygenase, measured by a simple microplate assay, was selected as an... |
| Tipo: Text |
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| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00100/21169/18787.pdf |
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| Burgeot, Thierry; Bocquene, Gilles; Truquet, Philippe; Le Dean, Loic; Poulard, Jean-charles; Dorel, Didier; Souplet, Arnauld; Galgani, Francois. |
| An oceanographic cruise in 1991 along the English Channel and Atlantic coasts of France indicated the value of dragonet Callionymus lyra Linnaeus, 1758 as a target species for monitoring biological effects. Ethoxyresorufin-O-deethylase measurements in this species provided a first assessment of the effects of hydrocarbons (PCB, PAH, dioxins) on the marine environment. The specific behavior of dragonet was defined by biochemical characterization. Biological data were obtained during 3 additional oceanographic cruises to study changes in the abundance and distribution of the species in the English Channel and the Bay of Biscay. This first assessment, carried out in the context of the National Observation Network, indicated that pollutants do not have... |
| Tipo: Text |
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| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00091/20243/17895.pdf |
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| Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne. |
| La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude. |
| Tipo: Text |
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| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf |
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| Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois. |
| Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),... |
| Tipo: Text |
Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf |
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| Beliaeff, Benoit; Burgeot, Thierry. |
| Activity of 7-ethoxyresorufin-O-deethylase (EROD) in fish is certainly the best-studied biomarker of exposure applied in the field to evaluate biological effects of contamination in the marine environment. Since 1991, a feasibility study for a monitoring network using this biomarker of exposure has been conducted along French coasts. Using data obtained during several cruises, this study aims to determine the number of fish required to detect a given difference between 2 mean EROD activities, i.e. to achieve an a priori fixed statistical power (l-beta) given significance level (alpha), variance estimations and projected ratio of unequal sample sizes (k). Mean EROD activity and standard error were estimated at each of 82 sampling stations. The... |
| Tipo: Text |
Palavras-chave: Biomonitoring; Estimation; EROD activity; Statistical power; Sample size; Sampling design. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00337/44787/44483.pdf |
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| Amiard-triquet, Claude; Burgeot, Thierry; Claisse, Didier. |
| Monitoring of marine environment quality can be based on chemical analysis of contaminants in the major components of the environment: seawater, sediment, biota. The respective interest of these different matrices is discussed and the fundamentals for the use of bivalves in the international Mussel Watch are presented. The objectives of the French RNO are 1) to assess the levels and trends of contaminants; 2) to assess the biological effects of contaminants. Analysis of the chemicals in biota is an indispensable complement to the biological approach, allowing their bioavailable fraction to be quantified. However, only a fraction of xenobiotics incorporated in living beings is biologically active and can exert a noxious effect on the biota. In addition,... |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00017/12848/9793.pdf |
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| Akcha, Farida; Burgeot, Thierry; Budzinski, H; Pfohla Leszkowicz, A; Narbonne, J. |
| With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg(-1) dry wt mussel d(-1)) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was... |
| Tipo: Text |
Palavras-chave: DNA repair; 8 oxodGuo; DNA adducts; Mussel; BaP. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-813.pdf |
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| Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois. |
| A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf |
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| Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
| As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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| Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F.. |
| In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was... |
| Tipo: Text |
Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf |
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| Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry. |
| Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and... |
| Tipo: Text |
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf |
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| Soletchnik, Patrick; Bouchet, Vincent; Malestroit, Pascal; Seugnet, Jean-luc; Blouin, Frederic; Radford-knoery, Joël; Burgeot, Thierry; Sauriau, Pierre-guy. |
| In the scope of the study of the "on-bottom/off-bottom" mortality model in the south of the Marennes Oléron Bay, the result of the 2003 multidisciplinary study (Dynamor) appears to be the hypothesis of an acute stress, "ultimate" trigger of the mortality episodes in June. In 2004 we aim to look for the nature of this stress through the physico-chemical analysis of the sediment (Eh, pH, sulphides and ammoniacal nitrogen), the organic matter and chlorophyll load on the surface of the sediment between April and July. Herbicides are also measured in the water column, south of the bay (Seudre), during the same period. A "precocious" (around the first week of June) and "weak" (lower mortality rate on this working site since 1997) mortality peak happens when the... |
| Tipo: Text |
Palavras-chave: Bassin Marennes Oléron; Phytobenthos; Mortalité; Crassostrea gigas; Foraminiferes benthiques; Répartition verticale; Sédiment; Huître; Echantillonnage. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-3383.pdf |
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| Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
| The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
| Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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| Registros recuperados: 62 | |
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